Micro- immunohistochemistry on cancerous breast tissue sections using different antibodies. (a) Monoplex staining of a 4-μm-thick well-differentiated invasive ductal carcinoma breast tissue section using a monoclonal mouse anti-human p53 protein antibody (54 μg ml-1, residence time 200 s). A line of hematoxylin is patterned with 1 s residence time for counterstaining. (b) Multiplexed staining of a well-differentiated invasive ductal carcinoma breast tissue section for the presence of p53 (α-p53: 54 μg ml-1, residence time of 300 s) and human progesterone receptor PR (α-PR: 125 μg ml-1, residence time of 300 s) with an additional hematoxylin counterstain. (c) μIHC processing of selected cores of an infiltrating ductal carcinoma breast tissue microarray, each 2 mm in diameter. Each core of the tissue array is characterized a priori by the supplier for the expression of ER, PR and p53. Four cores of the tissue microarray (locations A5, B5, B9 and C5) were processed with the vMFP (α-ER: 370 μg ml-1, α-PR: 125 μg ml-1 and α-p53: 54 μg ml-1).

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